一种采用火焰离子化检测的薄层色谱法对血浆脂质进行简单筛查的方法。
A simple screening method for plasma lipids by thin-layer chromatography with flame ionization detection.
作者信息
Vandamme D, Vankerckhoven G, Vercaemst R, Soetewey F, Blaton V, Peeters H, Rosseneu M
出版信息
Clin Chim Acta. 1978 Oct 16;89(2):231-8. doi: 10.1016/0009-8981(78)90321-2.
Thin-layer chromatography was carried out on glass rods coated with Silica gel (chromarod) and the fractions were detected with a flame ionization detector (FID). This system was applied to the fractionation and screening of plasma lipids. As the relative response of the flame ionization detector depends on the nature of the lipids, correction factors had to be introduced. These correction factors were derived from comparison of the results of the thin-layer chromatography with flame ionization detection with those obtained by conventional chemical analysis of the same sample. The coefficients of variation of the lipid profiles were comparable for both techniques. The absolute lipid concentration for the various fractions obtained by combination of the concentration of the total lipids with the relative lipid profile, correlate very well with the values obtained by conventional chemical analysis of the various lipids.
在涂有硅胶的玻璃棒（色谱棒）上进行薄层色谱分析，并用火焰离子化检测器（FID）检测各馏分。该系统应用于血浆脂质的分离和筛选。由于火焰离子化检测器的相对响应取决于脂质的性质，因此必须引入校正因子。这些校正因子是通过将薄层色谱与火焰离子化检测的结果与同一样品的常规化学分析结果进行比较而得出的。两种技术的脂质谱变异系数具有可比性。通过将总脂质浓度与相对脂质谱相结合得到的各种馏分的绝对脂质浓度，与通过各种脂质的常规化学分析得到的值相关性很好。
Zotero 插件