Orndorff S A, Colwell R R
Appl Environ Microbiol. 1980 Feb;39(2):398-406. doi: 10.1128/aem.39.2.398-406.1980.
Pseudomonas aeruginosa strain KO3 and a mixed aerobic enrichment culture, isolated from sewage sludge lagoon water, were found to aerobically transform the chlorinated insecticide Kepone, yielding monohydro-Kepone. Identification of the product was confirmed by gas chromatography and electron impact mass spectrometry. The mixed culture and P. aeruginosa strain KO3 produced about 4 and 16%, respectively, dihydro-Kepone, determined by cochromatography using authentic standards. Reduced amounts of monohydro-Kepone, compared with the mixed and pure cultures, were produced by James River sediment microorganisms. Kepone was not utilized as a sole carbon or energy source by any of the bacteria or mixed cultures examined in this study.
从污水污泥泻湖水中分离出的铜绿假单胞菌菌株KO3和一种混合好氧富集培养物，被发现可对氯化杀虫剂开蓬进行好氧转化，生成单氢开蓬。产物的鉴定通过气相色谱法和电子轰击质谱法得以确认。使用标准品共色谱法测定，混合培养物和铜绿假单胞菌菌株KO3分别产生了约4%和16%的二氢开蓬。与混合培养物和纯培养物相比，詹姆斯河沉积物微生物产生的单氢开蓬量有所减少。在本研究中检测的任何细菌或混合培养物都未将开蓬用作唯一的碳源或能源。
