Goldhaber S Z, Hennekens C H, Markisz J A, McNeil B J, Glynn M A, Bettmann M A, Schur P H
Am Rev Respir Dis. 1982 Aug;126(2):360-1. doi: 10.1164/arrd.1982.126.2.360.
Detection of plasma deoxyribonucleic acid (DNA) by counterimmunoelectrophoresis (CIE) has been proposed as a rapid, inexpensive screening test for the diagnosis of pulmonary embolism (PE). To test the sensitivity of plasma DNA detection for the diagnosis of PE, we obtained blood samples from 89 patients when they underwent lung scanning for suspected PE. Plasma was analyzed qualitatively for DNA by CIE, using antiserum that could detect as little as 0.2 micrograms DNA/ml. Among 16 patients with PE diagnosed by high probability lung scan, pulmonary angiography, or at autopsy, plasma DNA was detected in 3 patients--a sensitivity of 19%. Of 6 patients with indeterminate lung scans and a possible diagnosis of PE, 1 had detectable plasma DNA. None of the 67 patients without a diagnosis of PE had detectable plasma DNA, so the specificity was 100%. Thus, although detection of plasma DNA by CIE has many desirable features of a screening test, further studies are needed before this test can be recommended in routine screening for PE.
通过对流免疫电泳（CIE）检测血浆脱氧核糖核酸（DNA）已被提议作为一种快速、廉价的筛查试验用于诊断肺栓塞（PE）。为了测试血浆DNA检测对PE诊断的敏感性，我们在89例因疑似PE接受肺部扫描的患者中采集了血样。使用能检测低至0.2微克DNA/毫升的抗血清，通过CIE对血浆进行DNA定性分析。在通过高概率肺部扫描、肺血管造影或尸检诊断为PE的16例患者中，3例检测到血浆DNA，敏感性为19%。在6例肺部扫描结果不确定且可能诊断为PE的患者中，1例检测到可检测的血浆DNA。67例未诊断为PE的患者中均未检测到可检测的血浆DNA，因此特异性为100%。因此，尽管通过CIE检测血浆DNA具有筛查试验的许多理想特征，但在推荐该试验用于PE的常规筛查之前，还需要进一步研究。
