在弗瑞德白血病细胞的不均一核RNA中，信息序列不与聚腺苷酸相邻。

Message sequences are not adjacent to poly(A) in heterogeneous nuclear RNA of Friend leukemic cells.

作者信息

Mackedonski V V, McConkey E H

出版信息

Eur J Biochem. 1978 Oct;90(2):397-404. doi: 10.1111/j.1432-1033.1978.tb12617.x.

DOI:10.1111/j.1432-1033.1978.tb12617.x

Abstract

Hybridization of labeled RNA with excess amounts of unlabeled complementary DNA (cDNA) was used to investigate the location of cytoplasmic mRNA sequences in heterogenous nuclear RNA (hnRNA) of noninduced Friend leukemic cells. A heterologous hybrid between hnRNA and cytoplasmic cDNA was formed. Two homologous hybrids were also formed, one between poly(A)-containing mRNA and cytoplasmic cDNA, and the other between poly-(A)-containing hnRNA and nuclear cDNA. All hybrids were selected on hydroxyapatite columns after RNase treatment. The hybrids were further investigated for the presence of poly(A). No poly(A) was found in the heterologous hybrid, while both homologous hybrids contained poly(A). From these results we conclude that there exists a spacer nucleotide sequence between the poly(A) and the message sequences in hnRNA.

摘要

用标记的RNA与过量的未标记互补DNA（cDNA）杂交，以研究非诱导性Friend白血病细胞核不均一RNA（hnRNA）中细胞质mRNA序列的位置。形成了hnRNA与细胞质cDNA之间的异源杂种。还形成了两个同源杂种，一个是含poly（A）的mRNA与细胞质cDNA之间的杂种，另一个是含poly（A）的hnRNA与细胞核cDNA之间的杂种。在核糖核酸酶处理后，所有杂种都在羟磷灰石柱上进行选择。进一步研究杂种中是否存在poly（A）。在异源杂种中未发现poly（A），而两个同源杂种都含有poly（A）。从这些结果我们得出结论，在hnRNA的poly（A）和信息序列之间存在间隔核苷酸序列。

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