Herman R C
Biochemistry. 1979 Mar 6;18(5):916-20. doi: 10.1021/bi00572a029.
The message-sequence content of pulse-labeled poly(A)+ HeLa heterogenous nuclear RNA (hnRNA) has been examined by hybridizations to an excess of message cDNA. Control experiments show that the message cDNA accurately reflects the sequence distribution of the complex mixture of poly(A)+ messages present in the HeLa cytoplasm. Pulse-labeled poly(A)+ molecules in both the lamina-associated and shnRNA fractions contain message sequences, and approximately 65% of the poly(A)-adjacent hnRNA sequences are homologous to the 3' ends of mRNA. The majority of the pulse-labeled hnRNA molecules contain abundant message sequences. By use of these techniques it is also shown that some pulse-labeled polyadenylated message sequences are still synthesized in the presence of the adenosine analogue 5,6-dichloro-beta-D-ribofuranosylbenzimidazole under conditions where little or no new cytoplasmic mRNA is produced.
通过与过量的信使cDNA杂交，对脉冲标记的多聚腺苷酸[poly(A)]⁺ 海拉细胞异质核RNA（hnRNA）的信息序列内容进行了检测。对照实验表明，信使cDNA准确反映了海拉细胞胞质中存在的多聚腺苷酸[poly(A)]⁺ 信息复杂混合物的序列分布。核纤层相关和小分子hnRNA（shnRNA）组分中的脉冲标记多聚腺苷酸[poly(A)]⁺ 分子都含有信息序列，并且大约65%的多聚腺苷酸[poly(A)]相邻hnRNA序列与mRNA的3'末端同源。大多数脉冲标记的hnRNA分子含有丰富的信息序列。通过使用这些技术还表明，在几乎不产生或不产生新的胞质mRNA的条件下，在腺苷类似物5,6-二氯-β-D-呋喃核糖基苯并咪唑存在的情况下，仍会合成一些脉冲标记的多聚腺苷酸化信息序列。
