二价离子与转运RNA结合的意义及机制
Significance and mechanism of divalent-ion binding to transfer RNA.
作者信息
Guéron M, Leroy J L
出版信息
Biophys J. 1982 Jun;38(3):231-6. doi: 10.1016/S0006-3495(82)84553-0.
Abstract
Phosphorus NMR shows that divalent ions (manganese) bind to tRNA phosphates as to those of DNA or isolated phosphodiesters. The time for dissociation of a phosphate-divalent ion complex is in the microsecond range. For no single phosphate is the affinity to divalent ions greater than 10 times that of the average phosphate. It is often stated that a small number of strong binding sites exist and are structurally and functionally important. This concept originates from binding curves whose properties should, instead, be traced to the polyelectrolyte nature of nucleic acids. The 31P NMR data preclude the existence of strong sites to which divalent ions would bind very selectively. The Spectroscopic and crystallographic observations of sites for divalent ions do not in fact demonstrate selective binding to these sites.
摘要
磷核磁共振显示，二价离子（锰）与tRNA磷酸基团的结合方式与和DNA或分离的磷酸二酯的结合方式相同。磷酸二价离子复合物的解离时间在微秒范围内。没有任何一个磷酸基团对二价离子的亲和力比平均磷酸基团的亲和力大10倍以上。人们常说存在少量强结合位点，这些位点在结构和功能上很重要。这一概念源于结合曲线，而结合曲线的性质实际上应归因于核酸的聚电解质性质。31P核磁共振数据排除了存在二价离子会非常选择性结合的强位点的可能性。事实上，对二价离子结合位点的光谱学和晶体学观察并未证明对这些位点有选择性结合。
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