Ott G, Arnold L, Limmer S
Laboratorium für Biochemie, Universität Bayreuth, Germany.
Nucleic Acids Res. 1993 Dec 25;21(25):5859-64. doi: 10.1093/nar/21.25.5859.
Several RNA duplexes corresponding to the acceptor arms of different tRNAs have been analyzed with respect to their divalent metal ion binding capability by means of proton NMR spectroscopy using paramagnetic Mn2+ ions as probes. In particular, the role of GU wobble base pairs has been analyzed with reference to their potential for creating metal ion binding sites. It is shown that both the structural modifications induced by GU pairs in the A-RNA geometry and the sequence context seem to affect the metal ion binding capabilities.
利用顺磁性的Mn2+离子作为探针，通过质子核磁共振光谱法，对几种对应于不同tRNA受体臂的RNA双链体的二价金属离子结合能力进行了分析。特别是，参照GU摆动碱基对形成金属离子结合位点的可能性，对其作用进行了分析。结果表明，GU碱基对在A-RNA结构中引起的结构修饰以及序列背景似乎都影响金属离子的结合能力。
