Favila L, Howes E L, Taylor W A, Mitchison N A
Clin Exp Immunol. 1982 May;48(2):307-14.
Lymphocytes adoptively transferred from syngeneic immune donors protect mice against challenge with Herpes simplex virus type 1. Normal mice require transfer 3 x 10(7) spleen cells for protection. Sublethal irradiation (450 rad) decreases the number required five-fold. Lymphocytes from non-immune donors do not protect, and hyperimmunization does not enhance the protection efficiency of donors. The viral LD50 varies through a range 10(5)-fold during the period of recovery from this amount of radiation, but over the same period there is little variation in the number of cells required for protection. Nor is there much variation in this number between strains of mice naturally susceptible (CBA) and resistant (C57) to the virus. We conclude that natural resistance operates at a level of virus handling prior to operation of the lymphocyte system, perhaps at the generation of interferon. As few as 1.3 x 10(6) immune T lymphocytes can protect against challenge provided that they are transferred together with normal spleen cells. We conclude that primed lymphocytes act in co-operation with non-immune cells.
从同基因免疫供体中过继转移的淋巴细胞可保护小鼠免受1型单纯疱疹病毒的攻击。正常小鼠需要转移3×10⁷个脾细胞才能获得保护。亚致死剂量照射（450拉德）可使所需细胞数量减少五倍。来自非免疫供体的淋巴细胞无保护作用，过度免疫也不会提高供体的保护效率。在从这种辐射剂量恢复的期间，病毒半数致死量（LD50）在10⁵倍的范围内变化，但在同一时期，保护所需的细胞数量几乎没有变化。对该病毒天然易感（CBA）和有抗性（C57）的小鼠品系之间，这个数量也没有太大差异。我们得出结论，天然抗性在淋巴细胞系统发挥作用之前的病毒处理水平上起作用，可能是在干扰素产生时。只要与正常脾细胞一起转移，低至1.3×10⁶个免疫T淋巴细胞就能提供保护，使其免受攻击。我们得出结论，致敏淋巴细胞与非免疫细胞协同发挥作用。
