Yamamoto T, Okano H, Okawa T, Sato N, Okinaga S, Arai K
Nihon Naibunpi Gakkai Zasshi. 1978 Jul 20;54(7):848-54. doi: 10.1507/endocrine1927.54.7_848.
A rapid and simple R.I.A. method for serum unconjugated estriol in pregnancy had been studied for its usefulness as a routine obstetrical test to assess fetal well-being. Diluted serum samples or known amounts of standard estriol were applied with 125 I-estriol on pre-packed minicolumns of Sephadex G-25, followed by appropriately diluted antiserum. During incubation, the columns were counted in an Auto Well gamma-System to read the total counts (T). After a 90 minute incubation period, the columns were eluted with a buffer solution to remove the bound fraction, and the free estriol remaining in the columns was counted (F). Two quality control samples and sera from 74 pregnant women were analysed by this new method, and the results were compared to those obtained by the conventional radioimmunoassay method. The new column method is simple, specific and accurate. Results can be calculated within 4 hours after 30 sample sera are brought into the laboratory. Within and between assay coefficients, variations are 12.6 and 13.5%, respectively. This method correlates well with the results of the conventional method (r=0.7088, p less than 0.001), which requires almost 2 full days to process the same numbers of samples. Results of clinical cases as well as advantages of measuring free-circulating estriol in pregnancy are discussed.
一种用于检测孕期血清游离雌三醇的快速简便放射免疫分析法（R.I.A.），已作为一项评估胎儿健康状况的常规产科检查方法进行了实用性研究。将稀释后的血清样本或已知量的标准雌三醇与¹²⁵I-雌三醇一起加至预先装填有葡聚糖凝胶G-25的微型柱上，随后加入适当稀释的抗血清。在孵育期间，将微型柱置于自动伽马计数系统中计数以读取总计数（T）。孵育90分钟后，用缓冲液洗脱微型柱以去除结合部分，对微型柱中剩余的游离雌三醇进行计数（F）。用这种新方法分析了两个质量控制样本以及74名孕妇的血清，并将结果与通过传统放射免疫分析法获得的结果进行比较。新的柱法简便、特异且准确。30份样本血清进入实验室后4小时内即可计算出结果。批内和批间系数变异分别为12.6%和13.5%。该方法与传统方法的结果相关性良好（r = 0.7088，p小于0.001），而传统方法处理相同数量的样本几乎需要整整两天时间。文中还讨论了临床病例结果以及孕期检测游离循环雌三醇的优势。
