Haigh W G, Hofman L F, Barron E J
Clin Chem. 1980 Feb;26(2):309-12.
The column method of Christner and Fetter (Steroids 24: 327, 1974) has been modified to give a simple, rapid assay for unconjugated estriol in serum. Estriol is isolated from serum by being retained on a Sephadex column, while estriol conjugates and serum proteins are eluted. The sample and labeled estriol compete for antibody on the column. Antibody is eluted, removing proportional amounts of sample and labeled estriol. Our modifications include using stable serum-based standards, shortening the incubation to 10 min, complete removal of conjugates, and regeneration of the columns so that they can be used repeatedly. The assay, which can be completed in 2 h, has a mid-range interassay CV of 8.2%.
克里斯特纳和费特的柱法（《类固醇》24: 327, 1974）已被改进，以提供一种简单、快速的血清中未结合雌三醇测定方法。通过保留在葡聚糖凝胶柱上从血清中分离出雌三醇，而雌三醇结合物和血清蛋白则被洗脱。样品和标记雌三醇在柱上竞争抗体。抗体被洗脱，去除等量的样品和标记雌三醇。我们的改进包括使用稳定的基于血清的标准品，将孵育时间缩短至10分钟，完全去除结合物，以及使柱子再生以便可重复使用。该测定法可在2小时内完成，中间范围的批间变异系数为8.2%。
