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用于确定巴西副球孢子菌酵母型细胞活力的各种技术的比较。

Comparison of various techniques for determining viability of Paracoccidioides brasiliensis yeast-form cells.

作者信息

Restrepo A, Cano L E, de Bedout C, Brummer E, Stevens D A

出版信息

J Clin Microbiol. 1982 Jul;16(1):209-11. doi: 10.1128/jcm.16.1.209-211.1982.

DOI:10.1128/jcm.16.1.209-211.1982
PMID:7107858
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272325/
Abstract

The viability of Paracoccidioides brasiliensis yeast-form cells was determined by colony-forming units, direct fluorescent staining, and production of germ tubes in slide culture. The first procedure was unreliable and time consuming; the latter two showed better correlation with hemacytometer total cell counts and required significantly less time.

摘要

通过菌落形成单位、直接荧光染色以及玻片培养中芽管的产生来确定巴西副球孢子菌酵母型细胞的活力。第一种方法不可靠且耗时；后两种方法与血细胞计数器的总细胞计数显示出更好的相关性，并且所需时间明显更少。

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本文引用的文献

1
Growth of Paracoccidioides brasiliensis yeast phase in a chemically defined culture medium.巴西副球孢子菌酵母相在化学成分明确的培养基中的生长
J Clin Microbiol. 1980 Aug;12(2):279-81. doi: 10.1128/jcm.12.2.279-281.1980.
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Recovery of fungi from seeded sputum samples: effect of culture media and digestion procedures.
Rev Inst Med Trop Sao Paulo. 1981 Jul-Aug;23(4):178-84.
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Studies on plating efficiency and estimation of viability of suspensions of Paracoccidioides brasiliensis yeast cells.巴西副球孢子菌酵母细胞悬液的接种效率及活力评估研究。
Mycopathologia. 1980 Jul 1;71(2):73-83. doi: 10.1007/BF00440612.
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Growth curves and nucleic acids content of mycelial and yeast-like forms of Paracoccidioides brasiliensis.巴西副球孢子菌菌丝体和酵母样形态的生长曲线及核酸含量
Mycopathol Mycol Appl. 1970 Dec 4;41(3):203-10. doi: 10.1007/BF02051098.
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Growth curves of the yeast-like form of Paracocidioides bradiliensis.巴西副球孢子菌酵母样形态的生长曲线。
Sabouraudia. 1975 Mar;13 Pt 1:22-9.
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Determination of the growth curves of the mycelial and yeast forms of Paracoccidiodes brasiliensis.巴西副球孢子菌菌丝体和酵母形态生长曲线的测定。
Mycopathologia. 1976 Oct 22;59(3):163-9. doi: 10.1007/BF00627878.
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A cytological study of morphogenesis in Coccidioides immitis.粗球孢子菌形态发生的细胞学研究。
Sabouraudia. 1976 Jul;14(2):185-98.
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A new fluorescent viability test for fungi cells.
Mycopathologia. 1979 Feb 28;66(3):175-7. doi: 10.1007/BF00683967.