Kashino S S, Calich V L, Singer-Vermes L M, Abrahamsohn P A, Burger E
Departamento de Imunologia, Universidade de São Paulo, Brasil.
Mycopathologia. 1987 Aug;99(2):119-28. doi: 10.1007/BF00436916.
The yeast phase of ten P. brasiliensis isolates were studied to characterize their growth pattern, morphology and ultrastructure. Growth curves were determined after counts of total and viable fungi units (FU) during 20 days. Three growth patterns were observed: slow, reaching approximately 10-30 X 10(6) FU/tube (Pb 18, Pb 265 and PB 2); intermediate, reaching 60-150 X 10(6) FU/tube (IVIC Pb 9, IVIC Pb 267, Pb SN, Pb Vitor and Pb Campo Grande) and fast, reaching 180-370 X 10(6) FU/tube (Pb 2052 and Pb 192). The highest percentage of viable cells occurred on the 6th day of culture for Pb 192, Pb Campo Grande, Pb 2052 and IVIC Pb 9; on the 8th day for Pb Vitor, Pb SN, Pb 18 and IVIC Pb 267; on the 10th day for Pb 265 and on the 12th day of culture for Pb 2. Mean generation times varied from approximately 21.2 (Pb 2052) to 102.6 hours (Pb 265). The isolates showed similar morphology, except IVIC Pb 267 which did not present a typical yeast-phase at 35 degrees C and the two fast-growing isolates (Pb 2052 and Pb 192) that presented smaller cell sizes and less tendency to clump. The ultrastructure of the isolates was similar: the cell walls presented a width of 0.1 to 0.2 mu; the mitochondria presented few cristae and had equivalent patterns of distribution and morphology; the endoplasmic reticulum was scanty, presenting narrow cisternae; the vacuoles, empty or filled with electron-dense material, were numerous and two to five nuclei with pores were constantly observed.
对10株巴西副球孢子菌分离株的酵母相进行了研究，以表征其生长模式、形态和超微结构。在20天内对总真菌单位和活真菌单位（FU）进行计数后确定生长曲线。观察到三种生长模式：缓慢型，达到约10 - 30×10⁶FU/管（Pb 18、Pb 265和PB 2）；中间型，达到60 - 150×10⁶FU/管（IVIC Pb 9、IVIC Pb 267、Pb SN、Pb Vitor和Pb Campo Grande）；快速型，达到180 - 370×10⁶FU/管（Pb 2052和Pb 192）。Pb 192、Pb Campo Grande、Pb 2052和IVIC Pb 9在培养第6天活细胞百分比最高；Pb Vitor、Pb SN、Pb 18和IVIC Pb 267在第8天；Pb 265在第10天；Pb 2在培养第12天。平均世代时间从约21.2小时（Pb 2052）到102.6小时（Pb 265）不等。除IVIC Pb 267在35℃时未呈现典型酵母相，以及两个快速生长的分离株（Pb 2052和Pb 192）细胞尺寸较小且聚集倾向较小外，分离株显示出相似的形态。分离株的超微结构相似：细胞壁宽度为0.1至0.2μm；线粒体嵴较少，分布和形态模式相同；内质网稀少，呈现狭窄的池；空泡或充满电子致密物质的空泡众多，经常观察到有两到五个有孔的细胞核。
