Budkowska A, Karwowska S
J Immunol Methods. 1982;51(3):341-6. doi: 10.1016/0022-1759(82)90401-x.
A solid-phase enzyme-linked immunosorbent assay (ELISA) was developed for the detection of hepatitis B core antigen (HBcAg) in preparations of core particles isolated from hepatocyte nuclei and obtained from serum Dane particles. Comparison with counterelectrophoresis (CEP) showed that the sensitivity of ELISA is about 80-100 times greater than that of CEP. The enzyme immunoassay was applied to studies on conversion of HBcAg to HBeAg and it was shown that treatment of core particles with SDS and 2-ME leading to exposure of HBeAg determinants resulted in a complete loss of antigenic activity of HBcAg.
开发了一种固相酶联免疫吸附测定法（ELISA），用于检测从肝细胞核分离并从血清大球形颗粒获得的核心颗粒制剂中的乙型肝炎核心抗原（HBcAg）。与对流免疫电泳（CEP）比较表明，ELISA的灵敏度比CEP高约80-100倍。该酶免疫测定法用于研究HBcAg向HBeAg的转化，结果表明，用SDS和2-ME处理核心颗粒导致HBeAg决定簇暴露，从而导致HBcAg的抗原活性完全丧失。
