一种用于定量测定血浆中奎尼丁及其代谢物的高效液相色谱法：在奎尼丁-苯妥英药物相互作用研究中的应用。

An HPLC method for the quantitation of quinidine and its metabolites in plasma: an application to a quinidine-phenytoin drug interaction study.

作者信息

Pershing L K, Peat M A, Finkle B S

出版信息

J Anal Toxicol. 1982 May-Jun;6(3):153-6. doi: 10.1093/jat/6.3.153.

DOI:10.1093/jat/6.3.153

Abstract

A rapid and simple method is described for the separation of quinidine (Q), its metabolites 3-hydroxyquinidine (30HQ) and 2'oxoquinidinone (QD) as well as the quinidine salt contaminant, dihydroquinidine (DHQ) in plasma by high pressure liquid chromatography (HPLC). A silica column was used with methanol: 1 N ammonium nitrate: 2 N ammonium hydroxide (28:1:1 v/v) as the mobile phase to separate the compounds and a fluorescence spectrophotometer for detection. Application of this method is demonstrated by the analysis of human plasma samples collected from a volunteer during a phenytoin-quinidine drug interaction study.

摘要

本文描述了一种快速简便的方法，用于通过高压液相色谱（HPLC）分离血浆中的奎尼丁（Q）、其代谢物3-羟基奎尼丁（3OHQ）和2'-氧代奎尼丁酮（QD）以及奎尼丁盐污染物二氢奎尼丁（DHQ）。使用硅胶柱，以甲醇：1N硝酸铵：2N氢氧化铵（28:1:1 v/v）作为流动相来分离化合物，并使用荧光分光光度计进行检测。在苯妥英-奎尼丁药物相互作用研究期间，对一名志愿者采集的人体血浆样本进行分析，证明了该方法的应用。