Easter B R, Sutton D A, Drewes S E
Hoppe Seylers Z Physiol Chem. 1978 Sep;359(9):1229-36. doi: 10.1515/bchm2.1978.359.2.1229.
[A21-Desamido]insulin is the major product formed during mild acid hydrolysis of bovine insulin at low insulin concentration. The derivative was isolated by standard procedures and its purity established by isoelectric focusing, disc electrophoresis and electrophoresis on cellulose acetate strips. The identity of the acid-transformed derivative was determined as [A21-desamido]insulin by the action of carboxypeptidase A, using conditions under which a C-terminal aspartic acid residue would not be removed. The biological activity of this crystalline derivative was found to be 15.9 units/mg as measured by the mouse convulsion assay.
[A21-脱酰胺基]胰岛素是在低胰岛素浓度下对牛胰岛素进行温和酸水解时形成的主要产物。该衍生物通过标准程序分离，并通过等电聚焦、圆盘电泳和醋酸纤维素条电泳确定其纯度。在不会去除C末端天冬氨酸残基的条件下，利用羧肽酶A的作用，将酸转化衍生物鉴定为[A21-脱酰胺基]胰岛素。通过小鼠惊厥试验测得，这种结晶衍生物的生物活性为15.9单位/毫克。
