Drewes S E, Robinson H M, Gliemann J
Hoppe Seylers Z Physiol Chem. 1979 Jul;360(7):987-96. doi: 10.1515/bchm2.1979.360.2.987.
Reaction of insulin with 2-chloro- or 2-iodo-N-methylpyridinium iodide affords, amongst others, an insulin analogue modified at tha A14-tyrosine residue. Degradative procedures which pinpoint the exact position of modification are described. Highest yields of the derivative are obtained using the 2-iodo-N-methylpyridinium iodide as reagent. In the mouse convulsion assay this derivative retains 72% of its activity.
胰岛素与2-氯-或2-碘-N-甲基碘化吡啶反应，除其他产物外，还得到一种在A14-酪氨酸残基处被修饰的胰岛素类似物。文中描述了确定修饰确切位置的降解程序。使用2-碘-N-甲基碘化吡啶作为试剂可获得该衍生物的最高产率。在小鼠惊厥试验中，该衍生物保留了其72%的活性。
