Wadell G, Varsanyi T M
Infect Immun. 1978 Jul;21(1):238-46. doi: 10.1128/iai.21.1.238-246.1978.
Restriction site mapping of the genomes of eight different isolates of adenovirus serotype 7 (Ad7) has been performed with six different restriction endonucleases. In this analysis, 37 different restriction sites were localized. Three distinctly different cleavage patterns of the genomes of the Ad7 strains were observed. These strains could not be distinguished by serological techniques. The following three subtypes were defined on the basis of their restriction site patterns: the Ad7 prototype, represented by strain Gomen originally isolated from a case of pharyngitis; subtype Ad7a, represented by the Ad7 vaccine strain and strains isolated from undifferentiated respiratory disease and from a healthy carrier; and a third subtype of Ad7, represented by three strains which were isolated from Swedish patients, all having pronounced clinical symptoms indicating severe systemic infection. A comparison of the restriction site maps of the protype of Ad3 and the three subtypes of Ad7 indicated greater differences in the position of restriction sites between strains of Ad7 than between strains of the two serotypes. This technique is consequently recommended to obtain a more precise definition of distinct entities of viruses.
利用六种不同的限制性内切酶对腺病毒血清型7（Ad7）的八个不同分离株的基因组进行了限制性酶切位点图谱分析。在该分析中，定位了37个不同的限制性酶切位点。观察到Ad7菌株基因组的三种明显不同的切割模式。这些菌株无法通过血清学技术区分。根据其限制性酶切位点模式定义了以下三个亚型：Ad7原型，由最初从一例咽炎病例中分离出的戈门（Gomen）菌株代表；Ad7a亚型，由Ad7疫苗株以及从未分化呼吸道疾病患者和健康携带者中分离出的菌株代表；Ad7的第三个亚型，由从瑞典患者中分离出的三株菌株代表，所有这些菌株都有明显的临床症状，表明存在严重的全身感染。Ad3原型和Ad7三个亚型的限制性酶切位点图谱比较表明，Ad7菌株之间限制性酶切位点位置的差异大于这两种血清型菌株之间的差异。因此，推荐使用该技术来更精确地定义病毒的不同实体。
