Zambrano N Z, Montes G S, Shigihara K M, Sanchez E M, Junqueira L C
Acta Anat (Basel). 1982;113(1):26-38. doi: 10.1159/000145534.
The collagen arrangement of different cartilages of several mammals was studied by the Picrosirius-polarization method applied to tissue sections whose proteoglycans had been previously removed by enzymatic digestion. This procedure not only increases collagen birefringency but it also enhances the optimal resolution, permitting an accurate observation of the collagen disposition in this tissue. Our observations led to the conclusion that in the material studied at least five general patterns of collagen arrangement occur. When studied with the aid of electron microscopy, different cartilages showed variable values in their collagen fibril diameter. The results obtained suggest a greater variability in the collagen fibril diameters of those cartilages submitted to strong pressures.
通过将天狼星红偏振法应用于先前已通过酶消化去除蛋白聚糖的组织切片，研究了几种哺乳动物不同软骨的胶原排列。该方法不仅增加了胶原双折射，还提高了最佳分辨率，从而能够准确观察该组织中胶原的分布。我们的观察结果得出结论，在所研究的材料中至少出现了五种胶原排列的一般模式。借助电子显微镜研究时，不同软骨的胶原纤维直径显示出不同的值。所得结果表明，承受强大压力的那些软骨的胶原纤维直径具有更大的变异性。
