Hainer B L, Danylchuk P, Cooper J, Weart C W
Am J Obstet Gynecol. 1982 Sep 1;144(1):67-71. doi: 10.1016/0002-9378(82)90396-9.
An evaluation of the limulus amebocyte lysate assay for detection of gonococcal endotoxin in cervical secretions was undertaken in 48 women from an ambulatory population with a low incidence of gonococcal infection. When cervical secretions were diluted 1 : 2,000, positive limulus amebocyte lysate assay results were obtained in four women (100%) with culture-proved gonococcal infection, and negative results were obtained in 25 of 44 women (57%) with culture-negative specimens. The limulus amebocyte lysate assay was not sufficiently specific in a low-incidence, largely asymptomatic population to be recommended as a rapid diagnostic procedure (60 minutes) in such a group. Standardization of sampling technique, size, and reagent preparation and elimination of vaginal contamination of cervical secretions may produce a limulus amebocyte lysate test with more consistent performance characteristics in varied populations. Since the predictive value of a test varies with the prevalence of the disease in the populations tested, a test proposed for use in a population with a low likelihood of disease must be tested in such a population.
对48名门诊人群中淋病奈瑟菌感染率较低的女性进行了评估，以检测宫颈分泌物中淋病奈瑟菌内毒素的鲎试剂检测法。当宫颈分泌物稀释1:2000时，4名经培养证实为淋病奈瑟菌感染的女性（100%）鲎试剂检测结果呈阳性，44名培养结果为阴性的女性中有25名（57%）检测结果为阴性。在低感染率、大多无症状的人群中，鲎试剂检测法的特异性不足，因此不建议在此类人群中作为快速诊断方法（60分钟）使用。采样技术、样本量和试剂制备的标准化以及消除宫颈分泌物的阴道污染，可能会使鲎试剂检测法在不同人群中的性能特征更加一致。由于检测的预测价值会因所检测人群中疾病的患病率而异，因此建议在疾病可能性较低的人群中使用的检测方法必须在此类人群中进行测试。
