Spagna V A, Prior R B, Perkins R L
Am J Obstet Gynecol. 1980 Jul 1;137(5):595-9. doi: 10.1016/0002-9378(80)90702-4.
In an evaluation of the limulus lysate assay (LLA) as a method for detecting gonococcal endotoxin in cervical exudates diluted 1:800, positive LLA results were obtained from 17 of 18 patients (94%) with culture-proved gonococcal cervicitis, and negative results were obtained from 22 of 22 patients (100%) with culture-negative specimens. In vitro tests comparing the sensitivity of the LLA for Neisseria gonorrhoeae and other gram-negative organisms showed the LLA to be more sensitive in detecting N. gonorrhoeae (minimum sensitivity, 10(4) organisms per milliliter) than other commonly encountered urogenital gram-negative bacteria (minimum sensitivity, greater than 10(5) organisms per milliliter). Thus, in preliminary studies involving otherwise healthy women, the LLA appeared to correlate with bacteriologic methods for diagnosing gonococcal cervicitis and may aid in identifying nongonococcal cervicitis. In addition, the LLA was easy to perform, with test results available within an hour after the patient's initial examination.
在一项将鲎试剂检测法（LLA）作为检测宫颈分泌物中淋菌内毒素的方法的评估中，该分泌物稀释比例为1:800，18例经培养证实为淋菌性宫颈炎的患者中有17例（94%）LLA检测结果为阳性，22例培养结果为阴性标本的患者中22例（100%）LLA检测结果为阴性。比较LLA对淋病奈瑟菌和其他革兰阴性菌敏感性的体外试验表明，LLA检测淋病奈瑟菌（最低敏感性为每毫升10⁴个菌）比其他常见的泌尿生殖道革兰阴性菌（最低敏感性大于每毫升10⁵个菌）更敏感。因此，在涉及健康女性的初步研究中，LLA似乎与诊断淋菌性宫颈炎的细菌学方法相关，并且可能有助于识别非淋菌性宫颈炎。此外，LLA操作简便，在患者初次检查后一小时内即可获得检测结果。
