亮氨酸在饥饿大鼠生酮作用中的角色。
The role of leucine in ketogenesis in starved rats.
作者信息
Thomas L K, Ittmann M, Cooper C
出版信息
Biochem J. 1982 May 15;204(2):399-403. doi: 10.1042/bj2040399.
Abstract
The quantitative significance of the conversion in vivo of L-[U-14C]leucine to ketone bodies was determined in rats starved for 3 or 48 h. In animals starved for 3 h, 4.4% of ketone-body carbon is derived from the metabolism of leucine, and in rats starved for 48 h the corresponding value is 2.3%. This conversion occurs rapidly, and the specific radioactivity of ketone bodies in blood is maximal at 2 min after the intravenous injection of labelled leucine for both periods of starvation. The flux of leucine in the blood is 1.01 and 1.04 mumol/min per 100 g body wt. respectively for animals starved for 3 and 48 h. The specific radioactivity of blood ketone bodies was compared at 2 min after the injection of labelled leucine, lysine and phenylalanine. The specific radioactivity was 4-5 fold higher with leucine than with lysine or phenylalanine.
摘要
在饥饿3小时或48小时的大鼠中，测定了L-[U-¹⁴C]亮氨酸在体内转化为酮体的定量意义。在饥饿3小时的动物中，4.4%的酮体碳来自亮氨酸的代谢，而在饥饿48小时的大鼠中，相应的值为2.3%。这种转化迅速发生，在两个饥饿时间段内，静脉注射标记亮氨酸后2分钟，血液中酮体的比放射性达到最大值。对于饥饿3小时和48小时的动物，血液中亮氨酸的通量分别为每100克体重1.01和1.04微摩尔/分钟。在注射标记亮氨酸、赖氨酸和苯丙氨酸后2分钟，比较了血液酮体的比放射性。亮氨酸的比放射性比赖氨酸或苯丙氨酸高4 - 5倍。
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