Abrams M A, Cooper C
Biochem J. 1976 Apr 15;156(1):33-46. doi: 10.1042/bj1560033.
An acute intraperitoneal injection of ethanol (0.7 or 2.1g/kg body wt.) causes the reversible, dose-dependent accumulation of hepatic triglyceride in rats. By using a pulse of [14C]palmitate injected into a tail vein, it was found that ethanol (2.1g/kg)had no effect on the flux of unesterified fatty acid of serum (4.3mumol/min per 100g body wt.). However, either dose increased the fraction of the total flux going to liver from 0.16 to0.27 as rapidly as could be measured (30s), and it remained elevated until all ethanol had been cleared from the blood. The fraction of the total radioactivity in lipids of liver that was in triglyceride increased linearly for 1 h from 30 to 50% and there was a simultaneous decrease in phospholipid from 60 to 40%. The rate of synthesis of hepatic triglyceride derived directly from unesterified fatty acid of serum was calculated by using the flux rate of unesterified fatty acid in serum, the fractional hepatic uptake of this flux, and the percentage of liver fatty acid esterified to triglyceride. This contribution is related to the total synthetic rate of hepatic triglyceride (rate of accumulation+rate of release) to determine quantitatively how much of the developing fatty liver is attributable to increased uptake of unesterfied fatty acid of serum. At the higher dose of ethanol, about half of the accumulating triglyceride is derived from this source, whereas with the lower dose of ethanol it can account for all of the build-up.
腹腔内急性注射乙醇（0.7或2.1克/千克体重）会导致大鼠肝脏甘油三酯可逆性、剂量依赖性蓄积。通过向尾静脉注射[14C]棕榈酸脉冲，发现乙醇（2.1克/千克）对血清非酯化脂肪酸通量（每100克体重4.3微摩尔/分钟）没有影响。然而，两种剂量均能在可测量的最快时间（30秒）内将进入肝脏的总通量比例从0.16提高到0.27，并且在血液中所有乙醇清除之前一直保持升高。肝脏脂质中甘油三酯形式的总放射性比例在1小时内从30%线性增加到50%，同时磷脂比例从60%下降到40%。通过使用血清中非酯化脂肪酸的通量率、该通量的肝脏摄取分数以及肝脏脂肪酸酯化到甘油三酯的百分比，计算了直接源自血清非酯化脂肪酸的肝脏甘油三酯合成速率。这一贡献与肝脏甘油三酯的总合成速率（蓄积速率+释放速率）相关，以定量确定发育中的脂肪肝有多少可归因于血清非酯化脂肪酸摄取增加。在较高剂量的乙醇作用下，约一半蓄积的甘油三酯来源于此，而在较低剂量的乙醇作用下，它可以解释所有的蓄积。
