Lobov V P, Skripka L V
Mol Biol (Mosk). 1984 May-Jun;18(3):759-65.
Nucleotide sequence organization in the genome of maize has been studied using renaturation kinetics of DNA and S-1 nuclease digestion of the renatured products. Approximately 40% of the genome consists of single copy sequences, and 15% of these sequences are interspersed between repeated sequences and are approximately 1100 nucleotide pairs long. About 54% of the genome consists of repeated sequences. Six per cent of the genome consists of foldback sequences. These sequences are distributed through at least 44% of the genome. It was found using renaturation kinetics that the sum of foldback and highly repeated DNA fractions of Dobrudzhanko maize and inbred lines differ in the amount of DNA composing the fractions. Comparison of the DNA of the Dobrudzhanko maize and inbred lines by the method of DNA-DNA hybridization indicates strong differences in the amount of polynucleotide homologies between the Dobrudzhanko maize and the D1 inbred line on one hand and the A619 inbred line on the other hand.
利用DNA复性动力学和对复性产物进行S-1核酸酶消化的方法,对玉米基因组中的核苷酸序列组织进行了研究。基因组中约40%由单拷贝序列组成,其中15%的序列散布在重复序列之间,长度约为1100个核苷酸对。基因组约54%由重复序列组成。基因组的6%由回文序列组成。这些序列分布在至少44%的基因组中。利用复性动力学发现,多布鲁占科玉米和自交系的回文和高度重复DNA组分的总和在构成这些组分的DNA量上存在差异。通过DNA-DNA杂交方法对多布鲁占科玉米和自交系的DNA进行比较表明,一方面多布鲁占科玉米与D1自交系,另一方面与A619自交系之间,多核苷酸同源性的量存在显著差异。