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[小体鲟(Acipenser acipenser)基因组中核苷酸序列的组织]

[Organization of nucleotide sequences in the genome of the sterlet Acipenser acipenser].

作者信息

Vladychenskaia N S, Kedrova O S, Petrov N B

出版信息

Mol Biol (Mosk). 1980 Sep-Oct;14(5):986-100.

PMID:7421823
Abstract

Three fractions of repeated sequences with different thermal stability after reassociation and zero time and single copy fractions as well were isolated from a sterlet (acipenser acipenser) genome. Repeated sequences with different thermal stabilities are organized as homogeneous families not reassociating with one another when being repeatedly annealed. These fractions consist of the similar kinetic components; the exception is the slowly reassociating component which is found only in the low temperature fraction. The sterlet repeated sequences were also fractionated according to their length on agarose A-50 columns. The long (no less than 1350 nucleotides) sequences are heterogeneous by extent of their divergency, some part of them being highly divergent and contain sequences that are homologous to repeated sequences with low thermal stability. A suggestion is made that long and short repeated sequences in the sterlet genome are partly shared. The short (about 400-600 nucleotides) repeated sequences in the sterlet genome are interspersed within single copy ones.

摘要

从鲟鱼(Acipenser acipenser)基因组中分离出了复性后具有不同热稳定性的三个重复序列组分、零时组分和单拷贝组分。具有不同热稳定性的重复序列被组织成同源家族,当反复退火时它们彼此之间不会复性。这些组分由相似的动力学成分组成;例外的是仅在低温组分中发现的缓慢复性成分。鲟鱼的重复序列也在琼脂糖A - 50柱上根据其长度进行了分级分离。长(不少于1350个核苷酸)序列在其分歧程度上是异质的,其中一些部分高度分歧,并且包含与低热稳定性重复序列同源的序列。有人提出鲟鱼基因组中的长重复序列和短重复序列部分是共享的。鲟鱼基因组中的短(约400 - 600个核苷酸)重复序列散布在单拷贝序列之中。

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