Schmassmann A, Mikuz G, Bartsch G, Rohr H
Eur Urol. 1982;8(5):274-9. doi: 10.1159/000473535.
An approach was made to replace the subjective evaluation of human sperm morphology by an objective method applicable in routine laboratories. 20 patients out of infertile marriages (G2 and G3) were compared with 10 normal, fertile men (G1). Two different methods were used. Highly developed instruments and techniques were used in method 1, and cheap and simple ones, designed for routine laboratories, in method 2. Head area, circumference, length, and maximal midpiece width of 100 spermatozoa/subject were determined by a semi-automatic image analysis system. Head variation and average midpiece width were clearly bigger in G2 and G3 than in G1. As a result, formulae were developed, determining the pathological alteration of sperm head and midpiece for the individual semen. In addition, an objective classification into different sperm types was elaborated. Using the developed formulae, results of methods 1 and 2 are well in agreement. Thus, method 2 is recommended for routine laboratories, allowing an objective and reproducible morphology determination within 30 min.
人们尝试用一种适用于常规实验室的客观方法取代对人类精子形态的主观评估。将20对不育夫妇（G2和G3组）中的男性与10名正常、可育男性（G1组）进行比较。使用了两种不同的方法。方法1使用了高度先进的仪器和技术，方法2使用了为常规实验室设计的廉价且简单的仪器和技术。通过半自动图像分析系统测定每个受试者100条精子的头部面积、周长、长度和中段最大宽度。G2和G3组精子的头部变异和中段平均宽度明显大于G1组。因此，开发了公式，用于确定个体精液中精子头部和中段的病理改变。此外，还制定了对不同精子类型的客观分类方法。使用所开发的公式，方法1和方法2的结果高度一致。因此，推荐方法2用于常规实验室，它能在30分钟内实现客观且可重复的形态学测定。
