Dawson P A, Filipe M I
Histochem J. 1982 May;14(3):361-83. doi: 10.1007/BF01011850.
As part of our investigation on glycoprotein synthesis in pre-malignant colonic epithelium, changes in the uptake of [3H]galactose were studied at the ultrastructural level. Normal control mucosa from rectal biopsies of patients with no known gastro-intestinal disease and mucosa adjacent to carcinoma ('transitional' mucosa) from specimens resected for colo-rectal cancel were compared. These tissues were incubated in TC 199 medium containing [3H]galactose for various intervals of time and for pulse labelling. Silver grain distribution was statistically analysed. The results showed a reduction in the incorporation of the galactose by 'transitional' mucosa. The uptake by this mucosa was less uniform than normal and showed considerable peaking in the endoplasmic reticulum of the goblet cells and in the Golgi of the absorptive cells, suggesting a blockage or alteration in glycoprotein synthesis. The differences were most marked in the middle crypt (the region of differentiation) and in the upper crypt (the region of maturation).
作为我们对癌前结肠上皮细胞糖蛋白合成研究的一部分，我们在超微结构水平上研究了[³H]半乳糖摄取的变化。将无已知胃肠道疾病患者直肠活检的正常对照黏膜与因结直肠癌切除标本中癌旁的黏膜（“过渡性”黏膜）进行比较。将这些组织在含有[³H]半乳糖的TC 199培养基中孵育不同时间间隔以进行脉冲标记。对银颗粒分布进行统计学分析。结果显示“过渡性”黏膜中半乳糖掺入减少。该黏膜的摄取不如正常情况均匀，在杯状细胞的内质网和吸收细胞的高尔基体中有相当明显的峰值，提示糖蛋白合成存在阻滞或改变。差异在隐窝中部（分化区域）和隐窝上部（成熟区域）最为明显。
