Tseng L, Mazella J, Mann W J, Chumas J
J Clin Endocrinol Metab. 1982 Nov;55(5):1029-31. doi: 10.1210/jcem-55-5-1029.
The ability of human endometrium to synthesize estrogen from testosterone (T) was investigated. Normal and malignant endometrial specimens were incubated in a complete nutrient medium with 1 muC/ml (approximately 10 pmol/ml) of [3H]T for 20 hrs. Various estrogens: estrone (E1), estradiol (E2), estrone sulfate (E1S), and estradiol-3-sulfate (E2S) were isolated from cultured tissue and medium. The capacity of aromatization, expressed in pmol of estrogen formed/g of tissue, of proliferative endometria was found to be significantly higher than that of secretory endometria (prol. n=12, 0.53 +/- 0.21, vs sec. n=13, 0.15 +/- 0.09, mean +/- s.d., P less than 0.005). In nine cancer endometrial specimens studied, the estrogen produced varied from 0.3 to 15 pmol/g of tissue. These studies represent the first evidence that human endometrium is capable of synthesizing estrogens from delta 4 androgens at a concentration similar to plasma level. The changes of the capacity of aromatization during the two phases of the menstrual cycle indicate that the estrogen synthesis in endometrium is apparently regulated by hormones. The presence of aromatase in cancer endometria may play an important role in promoting the cell growth in estrogen sensitive endometrial cancer.
研究了人子宫内膜从睾酮（T）合成雌激素的能力。将正常和恶性子宫内膜标本在含有1μC/ml（约10pmol/ml）[3H]T的完全营养液中孵育20小时。从培养的组织和培养基中分离出各种雌激素：雌酮（E1）、雌二醇（E2）、硫酸雌酮（E1S）和硫酸雌二醇-3（E2S）。发现增殖期子宫内膜的芳香化能力（以每克组织形成的雌激素皮摩尔数表示）显著高于分泌期子宫内膜（增殖期n = 12，0.53±0.21，分泌期n = 13，0.15±0.09，平均值±标准差，P<0.005）。在研究的9个癌性子宫内膜标本中，产生的雌激素为每克组织0.3至15皮摩尔。这些研究首次证明人子宫内膜能够在与血浆水平相似的浓度下从δ4雄激素合成雌激素。月经周期两个阶段芳香化能力的变化表明子宫内膜中的雌激素合成明显受激素调节。癌性子宫内膜中芳香化酶的存在可能在促进雌激素敏感性子宫内膜癌的细胞生长中起重要作用。
