小鼠神经母细胞瘤细胞中神经节苷脂和唾液酸酶活性的密度依赖性变化。

Density-dependent changes in gangliosides and sialidase activity of murine neuroblastoma cells.

作者信息

Schengrund C L, Repman M A

出版信息

J Neurochem. 1982 Oct;39(4):940-7. doi: 10.1111/j.1471-4159.1982.tb11480.x.

DOI:10.1111/j.1471-4159.1982.tb11480.x

PMID:7119793

Abstract

Density-dependent changes in ganglioside composition, Vibrio cholerae neuraminidase (VCN)-susceptible sialyl residues, and membrane-associated sialidase activity were determined for the cholinergic murine neuroblastoma cell line S20Y. A decrease in total ganglioside sialic acid and VCN-releasable sialic acid was observed with increasing cell density. GM3 was the major ganglioside component of preconfluent S20Y cells, whereas GD1a was predominant in postconfluent cells. Sialidase activity increased in confluent and postconfluent cells and may account for the reduction in total ganglioside sialic acid observed with increasing cell density. In contrast, while adrenergic N115 cells showed a decrease in VCN-susceptible sialic acid residues with increasing cell density, there was no significant change in ganglioside composition or ganglioside sialic acid levels.

摘要

测定了胆碱能小鼠神经母细胞瘤细胞系S20Y中神经节苷脂组成、霍乱弧菌神经氨酸酶（VCN）敏感唾液酸残基以及膜相关唾液酸酶活性的密度依赖性变化。随着细胞密度增加，观察到总神经节苷脂唾液酸和VCN可释放唾液酸减少。GM3是汇合前S20Y细胞的主要神经节苷脂成分，而GD1a在汇合后细胞中占主导地位。汇合和汇合后细胞中的唾液酸酶活性增加，这可能解释了随着细胞密度增加总神经节苷脂唾液酸减少的现象。相比之下，虽然肾上腺素能N115细胞随着细胞密度增加VCN敏感唾液酸残基减少，但神经节苷脂组成或神经节苷脂唾液酸水平没有显著变化。