Vértes Z, Melegh G, Vértes M, Kovács S
Life Sci. 1982 Jul 12;31(2):119-26. doi: 10.1016/0024-3205(82)90423-4.
Effects of a single dose of naloxone and of D-Met2-Pro5-enkephalinamide on the DNA synthesis in the forebrain, hypothalamus and cerebellum of 11 day old female rats were studied. As an index of DNA synthesis the rate of incorporation of 3H-thymidine into DNA was measured 30 min after a sc. injection of 40 muCi/100 g b.w.. A time dependent effect of naloxone administration on cerebral DNA synthesis was observed. In the forebrain at 1 and 3 hrs after naloxone injection an increased rate of 3H-thymidine incorporation into DNA was found followed by a marked decrease at 9 and 12 hrs. The effect in the hypothalamus was similar but the initial increase at 1 hr was absent. On cerebellar DNA synthesis naloxone had no effect. The administration of D-Met2-Pro5-enkephalinamide resulted in a marked reduction in the labelling of cerebral and hypothalamic DNA between 1 to 12 hrs. Except a decrease at 1 hr no effect was found in the cerebellum.
研究了单剂量纳洛酮和D-蛋氨酸2-脯氨酸5-脑啡肽酰胺对11日龄雌性大鼠前脑、下丘脑和小脑DNA合成的影响。作为DNA合成的指标，在皮下注射40μCi/100g体重30分钟后，测定3H-胸腺嘧啶核苷掺入DNA的速率。观察到纳洛酮给药对脑DNA合成的时间依赖性效应。在纳洛酮注射后1小时和3小时，前脑中3H-胸腺嘧啶核苷掺入DNA的速率增加，随后在9小时和12小时显著下降。下丘脑中的效应相似，但1小时时没有初始增加。纳洛酮对小脑DNA合成没有影响。D-蛋氨酸2-脯氨酸5-脑啡肽酰胺的给药导致1至12小时期间脑和下丘脑DNA标记显著减少。除了1小时时减少外，在小脑中未发现影响。
