Ordög T, Vértes Z, Vértes M
Hungarian Academy of Sciences, Institute of Physiology, Pécs.
Life Sci. 1992;51(15):1187-96. doi: 10.1016/0024-3205(92)90355-s.
Opioid drugs and peptides were investigated for their effect on uterine DNA synthesis induced by a single injection of 17 beta-oestradiol given to ovariectomized rats 24 h prior to decapitation. [D-Met2,Pro5]-enkephalinamide administered 12, 2 or 1 h before killing resulted in a significant (approximately 50%) inhibition of in vitro [3H]-thymidine incorporation into DNA, while injections given 24 or 6 h before decapitation were ineffective. Non-linear regression of the dose-effect curves resulted in an ED50 of approximately 0.26 and approximately 0.45 microgram/100 g b.wt. for the opioid treatments given 12 or 2 h before killing, respectively. These effects could be completely reversed by the opioid antagonist naloxone injected 30 min prior to the agonist treatment, while naloxone itself had no effect. Morphine and [D-Ala2,D-Leu5]-enkephalin administered 12 h, as well as dynorphin A fragment 1-13 given 2 h before decapitation also inhibited oestradiol-induced uterine DNA synthesis. In ovariectomized animals without 17 beta-oestradiol priming no significant effect of [D-Met2,Pro5]-enkephalinamide or naloxone on [3H]TdR incorporation was found.
研究了阿片类药物和肽对子宫DNA合成的影响，该合成由在断头前24小时给去卵巢大鼠单次注射17β-雌二醇诱导。在处死前12、2或1小时给予[D-蛋氨酸2，脯氨酸5]-脑啡肽酰胺导致体外[3H]-胸苷掺入DNA受到显著（约50%）抑制，而在断头前24或6小时注射则无效。剂量-效应曲线的非线性回归得出，在处死前12或2小时给予阿片类药物处理的ED50分别约为0.26和0.45微克/100克体重。这些效应可通过在激动剂处理前30分钟注射阿片类拮抗剂纳洛酮完全逆转，而纳洛酮本身无作用。在断头前12小时给予吗啡和[D-丙氨酸2，D-亮氨酸5]-脑啡肽，以及在断头前2小时给予强啡肽A片段1-13也抑制了雌二醇诱导的子宫DNA合成。在未用17β-雌二醇预处理的去卵巢动物中，未发现[D-蛋氨酸2，脯氨酸5]-脑啡肽酰胺或纳洛酮对[3H]TdR掺入有显著影响。
