Ivanova E A
Ontogenez. 1982 Jul-Aug;13(4):430-5.
In the in vitro experiments the distribution of radioactivity was studied in the subcellular fractions of the reproductive system and muscles of the rat embryos on the 14-, 16- and 18th days and of the rabbit embryos on the 18th and 20-22nd days of development after the preincubation in the medium with 1 alpha, 2 alpha-3H(n)-testosterone (T-3H). The total consumption of T-3H per mg of tissue is higher in the reproductive system than in the muscles and attains the maximum in the rabbit on the 21st and in the rat on the 18th day of development. The ratio of radioactivity in the nuclear and cytoplasmic fractions per mg of protein changes at the critical period of somatic sex differentiation: in the rabbit between the 20th and 21st and in the rat between the 16th and 18th days of development. The radioactivity in the nuclear fraction is markedly higher than in the cytoplasmic one on the 21st day in the rabbit and on the 18th day in the rat and, on the contrary, lower at all preceding times. The radioactivity in the microsomal fraction is higher than in the cytosol. The distribution of radioactivity of 5 alpha-dihydro-1 alpha, 2 alpha(n)-testosterone (DHT-3H) was studied on the 20th and 21st days of development in the rabbit embryos. No changes in the nucleocytoplasmic ratio of radioactivity was found, but the radioactivity decreased in all fractions on the 21st day.
在体外实验中，研究了在含有1α,2α-3H(n)-睾酮（T-3H）的培养基中预孵育后，大鼠胚胎在发育第14、16和18天以及兔胚胎在发育第18和20 - 22天的生殖系统和肌肉亚细胞组分中的放射性分布。每毫克组织中T-3H的总消耗量在生殖系统中高于肌肉，在兔发育第21天和大鼠发育第18天达到最大值。在体细胞性别分化的关键时期，每毫克蛋白质的核组分和细胞质组分中的放射性比值发生变化：兔在发育第20和21天之间，大鼠在发育第16和18天之间。在兔的第21天和大鼠的第18天，核组分中的放射性明显高于细胞质组分，相反，在之前所有时间都较低。微粒体组分中的放射性高于胞质溶胶。研究了兔胚胎在发育第20和21天5α-二氢-1α,2α(n)-睾酮（DHT-3H）的放射性分布。未发现放射性核质比有变化，但在第21天所有组分中的放射性均下降。
