Ivanova E A
Ontogenez. 1986 Mar-Apr;17(2):200-5.
The dynamics of radioactivity in subcellular fractions of the reproductive system and muscle has been studied in the guinea pig embryos from the 21st day of development till birth after the preincubation in a medium containing 1 alpha,2 alpha-3H(n) testosterone (T-3H). The total uptake of T-3H per mg tissue is higher in the reproductive system than in the muscle and attains the highest values during the period of differentiation of target organs by the male type (30-33 days). The nucleocytoplasmic ratio of radioactivity is shifted towards its increase in the nucleus with maximum on the 41st-45th days and by the moment of birth. Radioactivity in the microsomal fraction is higher than in the cytosol (soluble proteins). From the 27th day of development on, when the hormone uptake becomes selective, the radioactivity in the cytosol from the reproductive organs is higher than in the muscle tissue. The data obtained suggest the importance of processes in microsomes for differentiation and organogenesis of reproductive system.
在含有1α,2α-3H(n)睾酮（T-3H）的培养基中预孵育后，对豚鼠胚胎从发育第21天直至出生期间生殖系统和肌肉亚细胞组分中的放射性动力学进行了研究。每毫克组织对T-3H的总摄取量在生殖系统中高于肌肉，并且在雄性类型靶器官分化期（30 - 33天）达到最高值。放射性的核质比在第41 - 45天以及出生时向细胞核中增加的方向偏移。微粒体组分中的放射性高于胞质溶胶（可溶性蛋白质）中的放射性。从发育第27天起，当激素摄取变得具有选择性时，生殖器官胞质溶胶中的放射性高于肌肉组织中的放射性。所获得的数据表明微粒体中的过程对生殖系统的分化和器官发生具有重要意义。
