Medugorac I
Res Exp Med (Berl). 1982;180(3):255-62. doi: 10.1007/BF01852298.
Left ventricular collagen of rats aged 1,4,8, and 24 months, as well as young and adult pigs and beef, was successively extracted with neutral salt and dilute acid solutions and limited pepsin digestion. The distribution of type I and III collagen molecules in pepsin-solubilized collagen was analyzed with sodium dodecyl sulphate polyacrylamide gel electrophoresis in the presence of 3.6 M urea, under nonreducing and reducing conditions. Yields of dilute-acid-soluble collagen ad neutral-salt-soluble collagen were extremely low (0.3-0.8%, and less than 0.3% of total starting collagen, respectively), indicating the presence of an extensive interchain and intermolecular cross-linking network in left ventricular tissue of young and mature mammals. Comparison of protein patterns in electrophoresis gels indicated that myocardial collagen consists primarily of type I collagen molecules. The components of type III collagen also occurred in all investigated preparations, in varying and consistently lower proportions. The ratio of type III to type I collagen changed during postnatal growth and aging.
分别用中性盐溶液、稀酸溶液以及有限度的胃蛋白酶消化法，依次提取1个月、4个月、8个月和24个月大的大鼠以及幼年和成年猪与牛的左心室胶原蛋白。在3.6M尿素存在的情况下，于非还原和还原条件下，采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法分析胃蛋白酶可溶解胶原蛋白中I型和III型胶原蛋白分子的分布情况。稀酸可溶胶原蛋白和中性盐可溶胶原蛋白的产量极低（分别为起始胶原蛋白总量的0.3 - 0.8%和不到0.3%），这表明幼年和成熟哺乳动物的左心室组织中存在广泛的链间和分子间交联网络。电泳凝胶中蛋白质图谱的比较表明，心肌胶原蛋白主要由I型胶原蛋白分子组成。III型胶原蛋白的成分也存在于所有研究样本中，但其比例各不相同且始终较低。III型与I型胶原蛋白的比例在出生后生长和衰老过程中发生了变化。
