Medugorac I, Jacob R
Cardiovasc Res. 1983 Jan;17(1):15-21. doi: 10.1093/cvr/17.1.15.
Collagen of the normal and hypertrophied rat left ventricle was successively extracted with neutral salt and dilute acid solutions, and pepsin digestion. The yield of dilute-acid soluble collagen was only 0.3 to 0.6% of total collagen; the solubility with neutral salt was even lower. Limited pepsin digestion permitted extraction of 50 to 65% of total collagen. The distribution of the various types of collagen molecules was analysed in pepsin-solubilised collagen in the presence of 3.6 mol . litre-1 urea with the aid of electrophoresis on polyacrylamide gels. In all samples of nonreduced and reduced left ventricular collagen of the rat, disc patterns of pepsin-soluble collagen revealed the occurrence of dimeric and trimeric components, as well as aggregates of higher molecular weight. Such observations suggest the presence of an extensive interchain and intermolecular cross-linking network. Electrophoretic analysis of nonreduced and reduced pepsin-solubilised collagen also revealed heterogeneity of left ventricular rat collagen due to its occurrence as a mixture of type I and type III collagen. The proportion of type I collagen molecule components was substantially higher than that of type III components in all investigated samples of rat left ventricular connective tissue. Postnatal growth, aging and myocardial hypertrophy may affect the ratio of type I to type III components.
对正常和肥大的大鼠左心室胶原蛋白依次用中性盐、稀酸溶液进行提取，并进行胃蛋白酶消化处理。稀酸可溶性胶原蛋白的产量仅占总胶原蛋白的0.3%至0.6%；中性盐的溶解度甚至更低。有限的胃蛋白酶消化可提取出50%至65%的总胶原蛋白。在3.6摩尔/升尿素存在的情况下，借助聚丙烯酰胺凝胶电泳分析胃蛋白酶溶解的胶原蛋白中各种类型胶原蛋白分子的分布。在大鼠左心室胶原蛋白的所有未还原和还原样本中，胃蛋白酶可溶性胶原蛋白的圆盘状图谱显示存在二聚体和三聚体成分以及更高分子量的聚集体。这些观察结果表明存在广泛的链间和分子间交联网络。对未还原和还原的胃蛋白酶溶解胶原蛋白进行电泳分析还发现，大鼠左心室胶原蛋白具有异质性，因为它是I型和III型胶原蛋白的混合物。在所有研究的大鼠左心室结缔组织样本中，I型胶原蛋白分子成分的比例明显高于III型成分。出生后生长、衰老和心肌肥大可能会影响I型与III型成分的比例。
