Medugorac I
Basic Res Cardiol. 1982 Nov-Dec;77(6):589-98. doi: 10.1007/BF01908312.
Left ventricular collagen of various mammals (cat, cow, dog, pig, and rat) was successively extracted with neutral salt, and dilute acid solutions, and limited pepsin digestion. The distribution of the various types of collagen molecules in pepsin-solubilized ventricular collagen was analyzed electrophoretically on SDS-polyacrylamide gels in the presence of 3.6 M urea. Yields of dilute-acid-soluble collagen were only 0.4 to 0.6% of the total ventricular collagen, and less than 0.1% with neutral salt solution. Approx. 55-90% of the total collagen was extracted by limited pepsin digestion. Disc patterns of pepsin-soluble collagen revealed the presence of dimeric and trimeric components, as well as higher-molecular-weight aggregates in all samples of nonreduced and reduced ventricular collagen. Taken together, these findings suggest the presence of an extensive interchain and intermolecular cross-linking network in left ventricular collagen. Comparison of electrophoretical disc gel patterns of reduced and nonreduced pepsin-solubilized collagen indicated that left ventricular collagen is heterogenous in nature, consisting of a mixture of type I and type III collagen. It was evident that primarily type I components occur in ventricular collagen. The components of type III collagen molecules occurred in all investigated left ventricles in varying and consistently appreciably lower amounts. The proportions of type III and type I collagen vary in left ventricular tissue of different species.
用中性盐、稀酸溶液和有限量的胃蛋白酶消化法依次提取各种哺乳动物（猫、牛、狗、猪和大鼠）的左心室胶原蛋白。在3.6 M尿素存在的情况下，通过SDS-聚丙烯酰胺凝胶电泳分析胃蛋白酶溶解的心室胶原蛋白中各种类型胶原蛋白分子的分布。稀酸可溶性胶原蛋白的产量仅占心室总胶原蛋白的0.4%至0.6%，用中性盐溶液提取的产量不到0.1%。约55%至90%的总胶原蛋白通过有限量的胃蛋白酶消化法提取。胃蛋白酶可溶性胶原蛋白的圆盘电泳图谱显示，在未还原和还原的心室胶原蛋白的所有样品中都存在二聚体和三聚体成分以及更高分子量的聚集体。综上所述，这些发现表明左心室胶原蛋白中存在广泛的链间和分子间交联网络。还原和未还原的胃蛋白酶溶解胶原蛋白的电泳圆盘凝胶图谱比较表明，左心室胶原蛋白本质上是异质的，由I型和III型胶原蛋白的混合物组成。很明显，心室胶原蛋白中主要是I型成分。III型胶原蛋白分子的成分在所有研究的左心室中含量不同且始终明显较低。不同物种左心室组织中III型和I型胶原蛋白的比例各不相同。
