MacDonell M T, Singleton F L, Hood M A
Appl Environ Microbiol. 1982 Aug;44(2):423-7. doi: 10.1128/aem.44.2.423-427.1982.
Nine chemically defined inoculation diluents, with compositions ranging from 0.85% NaCl to 35% marine salts, were used to evaluate the influence of diluent composition on the biochemical profiles of 30 marine and estuarine bacterial strains, including species of Vibrio, Aeromonas, Allomonas, and Photobacterium. Results demonstrated that a 20% marine salts diluent enabled the characterization of halophilic strains normally nonreactive by the API 20E system. Furthermore, the use of 20% marine salts showed that certain environmental isolates, identifiable as Vibrio parahaemolyticus by the recommended clinical inoculation procedure, were Vibrio vulnificus. An analysis of the profiles provided by the nine diluents indicates that the API 20E system, modified by the use of a diluent composed of 20% marine salts and incubated at 22 degrees C, can provide a reliable tool for the rapid characterization of marine and estuarine bacterial isolates.
使用了九种化学成分明确的接种稀释剂，其成分从0.85%氯化钠到35%海盐不等，以评估稀释剂成分对30株海洋和河口细菌菌株生化特征的影响，这些菌株包括弧菌属、气单胞菌属、异单胞菌属和发光杆菌属的菌种。结果表明，20%海盐稀释剂能够鉴定出通常用API 20E系统无反应的嗜盐菌株。此外，使用20%海盐表明，某些通过推荐的临床接种程序鉴定为副溶血性弧菌的环境分离株实际上是创伤弧菌。对这九种稀释剂提供的特征分析表明，通过使用由20%海盐组成的稀释剂并在22摄氏度下培养进行改良的API 20E系统，可以为快速鉴定海洋和河口细菌分离株提供可靠工具。
