用于鉴定肠杆菌科的多重检测微量方法系统的准确性评估。
Evaluation of accuracy of multitest micromethod system for identification of Enterobacteriaceae.
作者信息
Washington J A, Yu P K, Martin W J
出版信息
Appl Microbiol. 1971 Sep;22(3):267-9. doi: 10.1128/am.22.3.267-269.1971.
Abstract
The Analytab system of 20 biochemical tests for identification of Enterobacteriaceae was evaluated in parallel with conventional tests on 128 Enterobacteriaceae, 5 Aeromonas, and 1 Yersinia enterocolitica. The results of tests for H(2)S and indole production, citrate utilization, lysine and ornithine decarboxylase, arginine dihydrolase, nitrate reduction, beta-galactosidase, and fermentation of arabinose, rhamnose, mannitol, and glucose showed almost complete agreement between the two systems. Eighty-eight per cent of Enterobacteriaceae were correctly speciated with the Analytab system; on repeat testing with heavier inocula of organisms failing to ferment glucose initially, the proportion of Enterobacteriaceae correctly speciated became 93%.
摘要
对用于鉴定肠杆菌科细菌的包含20项生化检测的Analytab系统，与传统检测方法同时对128株肠杆菌科细菌、5株气单胞菌和1株小肠结肠炎耶尔森菌进行了评估。硫化氢和吲哚产生试验、柠檬酸盐利用试验、赖氨酸和鸟氨酸脱羧酶试验、精氨酸双水解酶试验、硝酸盐还原试验、β-半乳糖苷酶试验以及阿拉伯糖、鼠李糖、甘露醇和葡萄糖发酵试验的结果表明，这两种系统之间几乎完全一致。88%的肠杆菌科细菌通过Analytab系统被正确鉴定；对最初未能发酵葡萄糖的细菌接种量更大的样本进行重复检测时，肠杆菌科细菌被正确鉴定的比例达到了93%。
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