从一种永久性人类细胞系中分离出的D型逆转录病毒（PMFV）核糖核酸的特性分析。
Characterization of the ribonucleic acid of a type D retrovirus (PMFV) isolated from a permanent human cell line.
作者信息
Mothes E
出版信息
Arch Geschwulstforsch. 1982 May;52(3):191-7.
PMID:7125854
Abstract
The RNA of a type D retrovirus (PMFV) isolated from and propagated in a human cell ine has been characterized. Like other retroviruses PMFV contains a 60 to 70 S RNA as determined by glycerol gradient velocity sedimentation with marker RNAs. When denatured by heat the high-molecular-weight RNA prepared from fresh virus yielded subunits of about 30 S. As shown by poly(U)-Sepharose chromatography the 60 to 70 S RNA as well as the 30 S subunits contain a poly (A) stretch of approximately 80 to 200 nucleotides.
摘要
从人细胞系中分离并在其中繁殖的D型逆转录病毒（PMFV）的RNA已得到鉴定。与其他逆转录病毒一样，通过用标记RNA进行甘油梯度速度沉降测定，PMFV含有60至70S的RNA。当通过加热变性时，从新鲜病毒制备的高分子量RNA产生约30S的亚基。如聚（U）-琼脂糖凝胶色谱所示，60至70S的RNA以及30S亚基都含有一段约80至200个核苷酸的聚（A）序列。
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