Nerenberg C, Runkel R A, Matin S B
J Pharm Sci. 1978 Nov;67(11):1553-7. doi: 10.1002/jps.2600671116.
A simple radioimmunoassay was developed for the determination of oxfendazole in plasma. Oxfendazole N-1(3)-valerate was coupled to polylysine via a carbodiimide reaction, and antiserum was developed in rabbits after inoculation with oxfendazole--polylysine conjugate. The assay was developed so that oxfendazole could be measured directly in a 0.1-ml aliquot of diluted or undiluted plasma. With the developed procedure, 200 pg of oxfendazole/ml of plasma can be determined quantitatively. Cross-reactivity was determined for closely related compounds and metabolites. The method was used to determine plasma concentration--time profiles in dogs and calves.
开发了一种简单的放射免疫分析法用于测定血浆中的奥芬达唑。通过碳二亚胺反应将奥芬达唑N-1(3)-戊酸酯与聚赖氨酸偶联，用奥芬达唑-聚赖氨酸偶联物接种兔子后制备抗血清。该分析方法的开发使得可以直接在0.1 ml稀释或未稀释的血浆等分试样中测量奥芬达唑。采用所开发的方法，可定量测定血浆中200 pg/ml的奥芬达唑。测定了密切相关化合物和代谢物的交叉反应性。该方法用于测定犬和犊牛的血浆浓度-时间曲线。
