Palermo P J, Lundberg J B
J Pharm Sci. 1978 Nov;67(11):1627-9. doi: 10.1002/jps.2600671133.
A simultaneous programmed temperature GLC assay for the active ingredients in a topical antiseptic cream is described. The sample is extracted directly using a dimethyl sulfoxide solution of p-cresol, 4-chlorophenol, and 2-amino-4-phenylthiazole as internal standards for phenol, chloroxylenol, and lidocaine hydrochloride, respectively. The resulting solution is chromatographed by temperature programming on an OV-225 column from 90 to 225 degrees. The internal standard calculation is accomplished using peak heights or peak areas. The relative standard deviation of all assays is less than 2%.
描述了一种用于局部抗菌乳膏中活性成分的同步程序升温气相色谱分析方法。使用对甲酚、4-氯苯酚和2-氨基-4-苯基噻唑的二甲亚砜溶液直接提取样品，分别作为苯酚、氯二甲酚和盐酸利多卡因的内标物。所得溶液在OV - 225柱上通过程序升温从90℃至225℃进行色谱分析。内标计算通过峰高或峰面积完成。所有分析的相对标准偏差小于2%。
