Marzouki Z M, Coniglio J G
Biol Reprod. 1982 Sep;27(2):312-5. doi: 10.1095/biolreprod27.2.312.
The effect of essential fatty acid deficiency on lipids of separated rat Sertoli and germinal cells was determined at various time intervals after the animals were placed on a fat-free diet. Alterations in the fatty acid composition typical of essential fatty acid deficiency were noted in the Sertoli cells as well as in germinal cells as early as Days 9-14 on the fat-free diet. These changes included increased concentrations of oleic acid (18:1W9)2 and the appearance of 20:3W9. In some of the rats there were also decreases in linoleic (18:2W6) and arachidonic acids ((20:4W6), but no significant differences were found between Sertoli and germinal cells. Feeding a corn oil diet to rats previously maintained on a fat-free diet for 4 weeks reversed the changes in fatty acid composition of both Sertoli and germinal cells at the times studied. Lipid changes in phospholipids closely reflected those observed in the total lipids. These early changes in Sertoli cell lipids caused by an essential fatty acid deficiency may have important consequences since the Sertoli cell lipids caused by an essential fatty acid deficiency may have important consequences since the Sertoli cells play a significant role in the spermatogenic process.
在动物开始食用无脂饮食后的不同时间间隔，测定必需脂肪酸缺乏对分离的大鼠支持细胞和生精细胞脂质的影响。早在无脂饮食第9 - 14天，就注意到支持细胞和生精细胞中出现了典型的必需脂肪酸缺乏引起的脂肪酸组成变化。这些变化包括油酸（18:1W9）2浓度增加以及20:3W9的出现。在一些大鼠中，亚油酸（18:2W6）和花生四烯酸（20:4W6）也有所减少，但支持细胞与生精细胞之间未发现显著差异。给先前食用无脂饮食4周的大鼠喂食玉米油饮食，在所研究的时间点逆转了支持细胞和生精细胞脂肪酸组成的变化。磷脂中的脂质变化密切反映了总脂质中观察到的变化。必需脂肪酸缺乏引起的支持细胞脂质的这些早期变化可能具有重要后果，因为支持细胞在生精过程中起着重要作用。
