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乙酰胆碱诱发的解偶联作用限制了荧光素黄在胰腺腺泡细胞之间的通过。

Acetylcholine-evoked uncoupling restricts the passage of Lucifer Yellow between pancreatic acinar cells.

作者信息

Findlay I, Petersen O H

出版信息

Cell Tissue Res. 1982;225(3):633-8. doi: 10.1007/BF00214809.

DOI:10.1007/BF00214809
PMID:7127411
Abstract

Direct cell to cell movement of the fluorescent dye Lucifer Yellow CH (457 daltons) in exocrine acinar tissue is demonstrated by direct observation of living mouse pancreatic segments. Electrical uncoupling of pancreatic acinar cells by local application of a high concentration of acetylcholine significantly restricts cell to cell passage of the fluorescent dye. This result shows that a secretagogue can control direct movement of organic molecules between cells through junctional channels.

摘要

通过对活的小鼠胰腺节段进行直接观察,证实了荧光染料路西法黄CH(457道尔顿)在外分泌腺泡组织中的细胞间直接移动。通过局部应用高浓度乙酰胆碱使胰腺腺泡细胞发生电去偶联,可显著限制荧光染料在细胞间的通过。该结果表明,促分泌剂可通过连接通道控制有机分子在细胞间的直接移动。

相似文献

1
Acetylcholine-evoked uncoupling restricts the passage of Lucifer Yellow between pancreatic acinar cells.乙酰胆碱诱发的解偶联作用限制了荧光素黄在胰腺腺泡细胞之间的通过。
Cell Tissue Res. 1982;225(3):633-8. doi: 10.1007/BF00214809.
2
Electrical coupling and uncoupling of exocrine acinar cells.外分泌腺泡细胞的电偶联和解偶联
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3
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The extent of dye-coupling between exocrine acinar cells of the mouse pancreas. The dye-coupled acinar unit.小鼠胰腺外分泌腺泡细胞之间染料偶联的程度。染料偶联腺泡单位。
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Extent and modulation of junctional communication between pancreatic acinar cells in vivo.体内胰腺腺泡细胞间连接通讯的范围及调节
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Pancreatic acinar cells: ionic dependence of acetylcholine-induced membrane potential and resistance change.胰腺腺泡细胞:乙酰胆碱诱导的膜电位和电阻变化的离子依赖性
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Pancreatic acinar cells: effects of micro-ionophoretic polypeptide application on membrane potential and resistance.胰腺腺泡细胞:微量离子电泳施加多肽对膜电位和电阻的影响。
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引用本文的文献

1
The extent of dye-coupling between exocrine acinar cells of the mouse pancreas. The dye-coupled acinar unit.小鼠胰腺外分泌腺泡细胞之间染料偶联的程度。染料偶联腺泡单位。
Cell Tissue Res. 1983;232(1):121-7. doi: 10.1007/BF00222378.
2
Acetylcholine modulation of the conductance of intercellular junctions between rat lacrimal cells.乙酰胆碱对大鼠泪腺细胞间连接电导的调节作用
J Physiol. 1986 Aug;377:283-95. doi: 10.1113/jphysiol.1986.sp016187.
3
Blockage of cell-to-cell communication within pancreatic acini is associated with increased basal release of amylase.

本文引用的文献

1
Gap junctional communication.间隙连接通讯
Annu Rev Physiol. 1981;43:479-91. doi: 10.1146/annurev.ph.43.030181.002403.
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[Physiological importance of gap junctions].[间隙连接的生理重要性]
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Structural correlates of gap junction permeation.缝隙连接通透的结构相关性。
胰腺腺泡内细胞间通讯的阻断与淀粉酶基础释放增加有关。
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A simple method for embedding small specimens for photomicrography and sectioning following intracellular microiontophoresis of lucifer yellow CH.一种在荧光素黄CH进行细胞内微离子电泳后,对小标本进行包埋以用于显微摄影和切片的简单方法。
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Lucifer dyes--highly fluorescent dyes for biological tracing.鲁米诺染料——用于生物追踪的高荧光染料。
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Variations in tight and gap junctions in mammalian tissues.哺乳动物组织中紧密连接和缝隙连接的变化。
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Function of electrotonic junctions in embryonic and adult tissues.电突触在胚胎和成年组织中的功能。
Fed Proc. 1973 Jan;32(1):65-75.
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Permeable junctions.可渗透连接
Cold Spring Harb Symp Quant Biol. 1976;40:49-63. doi: 10.1101/sqb.1976.040.01.008.
10
Gap junctions. Structural changes after uncoupling procedures.缝隙连接。解偶联程序后的结构变化。
J Cell Biol. 1977 Mar;72(3):628-41. doi: 10.1083/jcb.72.3.628.