Siegert W, Fenyö E M, Klein G
Int J Cancer. 1977 Jul 15;20(1):75-82. doi: 10.1002/ijc.2910200113.
Cell membranes of Moloney lymphoma cells (YAC, of strain A origin) were solubilized by NP40. The antigenicity of the solubilized protein fraction was assayed by inhibition of the corresponding cytotoxic reaction against YAC target cells. The Moloney leukemia virus (MLV)-determined cell surface antigen (MCSA) was detected with mouse antisera, produced by the repeated inoculation of heavily irradiated YAC cells into syngeneic mice. Virion proteins gp71, p30, p15, p12 and p10 were identified with goat or rabbit antisera against purified Rauscher and Friend leukemia virus proteins. MCSA was found to bind to Con-A--Sepharose and was eluted by mannoside together with H-2A AND GP71. In contrast, p30, p12, p10 and part of p15 and p15(E), were not retained on the column and could be separated from MCSA. Passage of the glycoprotein fraction through Sephadex G-200 led to the separation of MCSA activity from gp71 and H-2A. MCSA eluted between the immunoglobulin (IgG) and the bovine serum albumin (BSA) size markers. MCSA could be also separated from the known viral proteins and from H-2 by velocity centrifugation in sucrose gradients. It sedimented with approximately 6.6 S ahead of gp71 (4.4 S) and H-2 (3.2 S). It is suggested that MCSA may be a glycoprotein with an approximate molecular weight of 110,000 and distinct from the known viral proteins gp71, p30, p15(E), p12, p10 and from H-2.
莫洛尼淋巴瘤细胞（源自A品系的YAC细胞）的细胞膜用NP40进行溶解。通过抑制针对YAC靶细胞的相应细胞毒性反应来检测溶解蛋白组分的抗原性。用经多次将大量辐照的YAC细胞接种到同基因小鼠体内产生的小鼠抗血清检测莫洛尼白血病病毒（MLV）决定的细胞表面抗原（MCSA）。用针对纯化的劳斯氏和弗瑞德白血病病毒蛋白的山羊或兔抗血清鉴定病毒粒子蛋白gp71、p30、p15、p12和p10。发现MCSA与伴刀豆球蛋白A - 琼脂糖结合，并与H - 2A和gp71一起被甘露糖苷洗脱。相比之下，p30、p12、p10以及部分p15和p15（E）不保留在柱上，可与MCSA分离。糖蛋白组分通过葡聚糖G - 200导致MCSA活性与gp71和H - 2A分离。MCSA在免疫球蛋白（IgG）和牛血清白蛋白（BSA）大小标志物之间洗脱。通过在蔗糖梯度中进行速度离心，MCSA也可与已知病毒蛋白和H - 2分离。它沉降时约为6.6 S，先于gp71（4.4 S）和H - 2（3.2 S）。提示MCSA可能是一种分子量约为110,000的糖蛋白，与已知病毒蛋白gp71、p30、p15（E）、p12、p10以及H - 2不同。
