Sikora K, Koch G, Brenner S, Lennox E
Br J Cancer. 1979 Dec;40(6):831-8. doi: 10.1038/bjc.1979.273.
Plasma membranes isolated from two immunogenic, non-cross-protecting, MC sarcomas were shown to retain the specific rejection antigens of whole cells as well as serologically detected H-2 antigens. Solubilization of the membranes with sodium deoxycholate gave quantitative release of H-2 and retained the rejection specificity of the tumour from which it was derived. Polyacrylamide-gel electrophoresis (PAGE) showed no extensive degradation of membrane components during solubilization. The solubilized TSTAs were further characterized and purified on columns of 4 different lectins immobilized on sepharose beads. TSTA from both tumours bound to WGA but not to Con A, LCH or RCA columns. Specific activity was retained after elution from the WGA column. Serologically detectable H-2 bound to the Con A and LCH columns only. Clear separation of H-2 from TSTA activity was thus obtained. Furthermore the WGA-binding material represents a source for further purification of TSTA molecules in order to explore the basis for their diversity.
从两种具有免疫原性、无交叉保护作用的MC肉瘤中分离出的质膜，被证明保留了全细胞的特异性排斥抗原以及血清学检测到的H-2抗原。用脱氧胆酸钠溶解膜可定量释放H-2，并保留其来源肿瘤的排斥特异性。聚丙烯酰胺凝胶电泳（PAGE）显示，溶解过程中膜成分没有广泛降解。溶解的肿瘤特异性移植抗原（TSTA）在固定于琼脂糖珠上的4种不同凝集素柱上进一步进行表征和纯化。来自两种肿瘤的TSTA与小麦胚芽凝集素（WGA）结合，但不与刀豆球蛋白A（Con A）、扁豆凝集素（LCH）或蓖麻凝集素（RCA）柱结合。从WGA柱洗脱后仍保留了比活性。血清学可检测到的H-2仅与Con A和LCH柱结合。从而实现了H-2与TSTA活性的清晰分离。此外，WGA结合物质是进一步纯化TSTA分子的来源，以便探索其多样性的基础。
