Lee W, Hamernyik P, Hutchinson M, Raisys V A, Labbé R F
Clin Chem. 1982 Oct;28(10):2165-9.
Measurements of ascorbic acid concentration in leukocytes by "high-performance" liquid chromatography (HPLC) provides better nutritional assessment, leading to better management, particularly of presymptomatic and critically ill patients. This procedure includes a simple, reproducible cell-separation technique that requires no more than 2 mL of whole blood. Cell populations are separable with greater than 95% purity and greater than 99% viability. Ascorbic acid is assayed by HPLC. The vitamin can be reproducibly quantified in concentrations as low as 0.1 microgram/mL of cell extract. The chromatographic procedure is very rapid, analysis being completed within 15 min after specimen preparation. The assay is suitable also for urine and protein-free filtrates of plasma and of other biological materials. Reference intervals for plasma, mononuclear leukocytes, and polymorphonuclear leukocytes were established. A preliminary clinical evaluation revealed that hospital patients were at a greater risk of ascorbic acid deficiency than expected.
采用“高效”液相色谱法（HPLC）测定白细胞中的抗坏血酸浓度，能提供更好的营养评估，从而实现更好的管理，尤其是对无症状和重症患者。该程序包括一种简单、可重复的细胞分离技术，所需全血不超过2 mL。细胞群体的纯度可分离至95%以上，活力大于99%。通过HPLC测定抗坏血酸。该维生素在细胞提取物中的浓度低至0.1微克/毫升时仍可重复定量。色谱分析过程非常迅速，标本制备后15分钟内即可完成分析。该测定方法也适用于尿液以及血浆和其他生物材料的无蛋白滤液。已确定血浆、单核白细胞和多形核白细胞的参考区间。初步临床评估显示，住院患者患抗坏血酸缺乏症的风险比预期更高。
