Omaye S T, Schaus E E, Kutnink M A, Hawkes W C
Ann N Y Acad Sci. 1987;498:389-401. doi: 10.1111/j.1749-6632.1987.tb23776.x.
The fact that platelets, PMN leukocytes, and MN leukocytes concentrate ascorbic acid suggests that vitamin C has an important role in their physiological functions. The question still remains as to which one of the cells best reflects vitamin C status. The ascorbic acid content of PMNs and platelets correlates positively with plasma concentration and supplementation with vitamin C, as shown in Evans et al. They also found that MN leukocytes, in contrast, do not show any such relationship; however, MN leukocytes maintain the highest levels of ascorbic acid and play a very important function in immunocompetence. We have found that with a limited number of subjects, ascorbic acid content of MN and PMN leukocytes correlates positively with plasma ascorbic acid, but there was no correlation between platelets and plasma ascorbic acid (unpublished results). Therefore, further work is necessary to evaluate these three blood components for the best cellular marker of vitamin C status. We have developed a reversed-phase HPLC method for ascorbic acid that can be used in conjunction with our cellular differential centrifugation technique for the determination of ascorbic acid in relatively pure blood cell fractions. The chromatographic method is simple, sensitive, and automated. It clearly resolves ascorbic acid, which is the major form of the vitamin found in vivo and is not prone to interference by sugars, carbohydrates, or nucleotides.
血小板、中性粒细胞和单核细胞会富集抗坏血酸，这一事实表明维生素C在它们的生理功能中起着重要作用。然而，哪种细胞最能反映维生素C的状态仍是个问题。正如埃文斯等人所表明的，中性粒细胞和血小板中的抗坏血酸含量与血浆浓度以及维生素C补充量呈正相关。他们还发现，相比之下，单核细胞并未表现出任何此类关系；然而，单核细胞维持着最高水平的抗坏血酸，并且在免疫能力方面发挥着非常重要的作用。我们发现，在受试者数量有限的情况下，单核细胞和中性粒细胞中的抗坏血酸含量与血浆抗坏血酸呈正相关，但血小板与血浆抗坏血酸之间没有相关性（未发表的结果）。因此，有必要进一步开展工作，以评估这三种血液成分作为维生素C状态的最佳细胞标志物的情况。我们已经开发出一种用于抗坏血酸的反相高效液相色谱法，该方法可与我们的细胞差速离心技术结合使用，用于测定相对纯净的血细胞组分中的抗坏血酸。该色谱方法简单、灵敏且自动化。它能清晰地分离抗坏血酸，抗坏血酸是体内发现的维生素的主要形式，不易受到糖、碳水化合物或核苷酸的干扰。
