Ohtsuru M, Ishii Y, Takai S, Higashi H, Kosaki G
Cancer Res. 1980 Feb;40(2):477-80.
Peripheral lymphocytes from cancer patients receiving mitomycin C treatment were examined for cytogenetic effects. The treatment consisted of i.v. injections of mitomycin C at a dose of 4 mg given twice a week for 2 weeks. The lymphocytes were cultured in vitro for 72 hr with phytohemagglutinin and 5-bromodeoxyuridine, and then sister chromatid exchanges were scored. Before treatment with mitomycin C, the frequencies of sister chromatid exchanges in lymphocytes of cancer patients were similar to those of healthy controls. After the first and second treatments in vivo with mitomycin C, the frequencies of sister chromatid exchnages increased with time, reached a peak in about 24 hr, and then returned to the pretreatment level in about 48 hr, in contrast to the case of in vitro exposure to mitomycin C. After the third and fourth injections, however, the frequency increased further and did not return to the original level. The significance of the specific kinetics of change in the sister chromatid exchnage frequency after in vivo treatments is discussed in relation to cancer chemotherapy.
对接受丝裂霉素C治疗的癌症患者的外周淋巴细胞进行细胞遗传学效应检测。治疗方案为静脉注射丝裂霉素C，剂量为4毫克，每周两次，共2周。淋巴细胞在体外与植物血凝素和5-溴脱氧尿苷一起培养72小时，然后对姐妹染色单体交换进行计数。在用丝裂霉素C治疗前，癌症患者淋巴细胞中姐妹染色单体交换的频率与健康对照者相似。在体内进行第一次和第二次丝裂霉素C治疗后，姐妹染色单体交换的频率随时间增加，在约24小时达到峰值，然后在约48小时恢复到治疗前水平，这与体外暴露于丝裂霉素C的情况不同。然而，在第三次和第四次注射后，频率进一步增加且未恢复到原始水平。文中结合癌症化疗讨论了体内治疗后姐妹染色单体交换频率变化的特定动力学的意义。
