Brown M, Weber J
J Gen Virol. 1982 Sep;62 (Pt 1):81-9. doi: 10.1099/0022-1317-62-1-81.
Discrete subgenomic DNA fragments were found in three out of thirty-two preparations of adenovirus type 2 incomplete particles grown in human Hep-2 cells and examined over the course of 1 year. One preparation contained three fragments corresponding to 5, 14 and 19% of the genome, another contained a 37% fragment and the third a 40% fragment. Each fragment hybridized exclusively to the left end of the genome. Digestion of the nick-translated 37% fragment with HindIII confirmed that it contained the left 37.3% of the genome. Synthesis of these fragments was not dependent on high input multiplicity of infection. Comparable fragments were not found in unpackaged DNA from the corresponding infected cells. This is consistent with the hypothesis that such fragments are generated during virus assembly or, alternatively, may reflect the very small proportion of these fragments relative to the pool of unpackaged DNA within the cells. The possibility that they are generated by errors in DNA replication is discussed.
在人Hep-2细胞中培养并历经1年检测的32份2型腺病毒不完全颗粒制剂中，发现了3份含有离散的亚基因组DNA片段。一份制剂含有对应基因组5%、14%和19%的3个片段，另一份含有一个37%的片段，第三份含有一个40%的片段。每个片段仅与基因组的左端杂交。用HindIII消化缺口平移的37%片段证实，它包含基因组左侧的37.3%。这些片段的合成不依赖于高感染复数。在相应感染细胞的未包装DNA中未发现类似片段。这与以下假设一致，即此类片段是在病毒组装过程中产生的，或者可能反映了这些片段相对于细胞内未包装DNA库的比例非常小。文中讨论了它们由DNA复制错误产生的可能性。
