用快原子轰击质谱法对寡脱氧核糖核苷酸进行超快测序
Ultrafast sequencing of oligodeoxyribonucleotides by FAB-mass spectrometry.
作者信息
Grotjahn L, Frank R, Blöcker H
出版信息
Nucleic Acids Res. 1982 Aug 11;10(15):4671-8. doi: 10.1093/nar/10.15.4671.
Abstract
A fully instrumental method is described for the bidirectional sequencing of oligodeoxyribonucleotides. The method makes use of the negative ion fragmentation patterns of fast atom bombardment mass spectrometry. It is less time consuming than any other sequencing procedure known to date. Since one sequencing run takes as little as one hour, this new method is anticipated to cut down considerably the time required for the controlled synthesis of oligodeoxyribonucleotides of (currently) up to ten nucleotide units in length.
摘要
本文描述了一种用于寡脱氧核糖核苷酸双向测序的全仪器化方法。该方法利用了快原子轰击质谱的负离子裂解模式。它比迄今已知的任何其他测序方法都更省时。由于一次测序运行只需一小时，预计这种新方法将大大缩短（目前）长度达十个核苷酸单位的寡脱氧核糖核苷酸受控合成所需的时间。
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本文引用的文献
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