诺维科夫肝癌细胞对水和一元醇的渗透性。

Permeability of Novikoff hepatoma cells to water and monohydric alcohols.

作者信息

Polefka T G, Redwood W R, Garrick R A, Chinard F P

出版信息

Biochim Biophys Acta. 1981 Mar 20;642(1):67-78. doi: 10.1016/0005-2736(81)90138-3.

Abstract

The permeability coefficients of Novikoff hepatoma ascites cell membranes for tritiated water (3HHO) and for a homologous series of monohydric alcohols (methanol through hexanol) were deduced from linear diffusion coefficients by means of a series-parallel pathway model (Redwood et al. (1974) J. Gen. Physiol. 64, 706-729). Membrane permeability coefficients for 3HHO at 20, 30 and 37 degrees C were (all x 10(-5)) 97, 125, and 163 cm . s-1, respectively, and were significantly smaller than the corresponding values for the alcohols tested. In the alcohols series, ethanol had the lowest permeability coefficient 198 x 10(-5) cm . s-1 at 20 degrees C. The apparent activation energy for water permeation was 6.7 +/- 1.9 S.E. kcal . mol-1. The apparent membrane diffusion coefficients for the alcohols were a complex function of molecular properties with less diffusional membrane resistance to the alcohols in the middle of the homologous series than would have been expected on the basis of oil-water partitioning or molar volume considerations. The conventional parallel aqueous lipophilic pathway model is not consistent with the present data which can be interpreted by consideration of parallel lipophilic pathways through the Novikoff hepatoma cell membrane.

摘要

利用串并联途径模型（Redwood等人，（1974年）《普通生理学杂志》64卷，706 - 729页），从线性扩散系数推导出诺维科夫肝癌腹水细胞膜对氚水（³HHO）和一系列同系一元醇（从甲醇到己醇）的渗透系数。³HHO在20℃、30℃和37℃时的膜渗透系数（均×10⁻⁵）分别为97、125和163 cm·s⁻¹，且明显小于所测试醇类的相应值。在醇类系列中，乙醇在20℃时的渗透系数最低，为198×10⁻⁵ cm·s⁻¹。水渗透的表观活化能为6.7±1.9标准误 kcal·mol⁻¹。醇类的表观膜扩散系数是分子性质的复杂函数，同系物系列中间的醇类对膜的扩散阻力比基于油水分配或摩尔体积考虑所预期的要小。传统的平行水相 - 亲脂途径模型与目前的数据不一致，通过考虑穿过诺维科夫肝癌细胞膜的平行亲脂途径可以解释这些数据。